protoarray human protein micro array v5 0 (Thermo Fisher)
Structured Review

Protoarray Human Protein Micro Array V5 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
Images
1) Product Images from "Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD"
Article Title: Autoantibody Profiles in Collagen Disease Patients with Interstitial Lung Disease (ILD): Antibodies to Major Histocompatibility Complex Class I-Related Chain A (MICA) as Markers of ILD
Journal: Biomarker Insights
doi: 10.4137/BMI.S28209
Figure Legend Snippet: Auto-Ab identification methods used in this study. ( A ) Sera from the 34 rheumatoid arthritis (RA) patients with or without ILD were analyzed for anti-HLA Ab profiles. ( b ) Sera from the 15 collagen disease patients with acute-onset diffuse ILD (AoDILD) were collected on admission and in the stable state. Two samples were collected from each patient. These individual sera were analyzed for anti-HLA Ab profiles or granulocyte immunofluorescence test (GIFT). ( C ) The sera from these patients either with AoDILD or in the stable state were combined; the two pooled sera at these two states were screened for the auto-Ab profiling in ProtoArray and AlphaScreen systems. ( D ) These individual sera were analyzed by glutathione S-transferase (GST) capture enzyme-linked immunosorbent assay (ELISA). Analyses of individual sera are written in black and those of pooled sera in red.
Techniques Used: Immunofluorescence, Amplified Luminescent Proximity Homogenous Assay, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: Auto-Abs present in the pooled sera from collagen disease patients with acute-onset diffuse ILD (AoDILD). The numbers of auto-Abs detected with ( A ) ProtoArray and ( b ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle). The numbers of auto-Abs for proteins overlapped between ( C ) ProtoArray and ( D ) AlphaScreen in pooled sera from patients with collagen disease in the stable (left circle) and AoDILD states (right circle).
Techniques Used: Amplified Luminescent Proximity Homogenous Assay